LOCUS       40924_23859       12841 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB754 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12841)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12841)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12841)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2874..2894)
                     /label=attB2
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             2906..3580
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3605..3857
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3891..3907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3915..3931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3939..3969)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3984..4005)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4041..4293)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4331..5626)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61289.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4331..5626)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5678..5857)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6363..6387)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6908..7696
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7945..8533
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8719..8859)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9203..9397)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9466..10536)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10968..11594)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcatggc ctcctccgag gacgtcatca aggagttcat
     2941 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3001 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     3061 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc cagtacggct ccaaggccta
     3121 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     3181 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3241 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     3301 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc ggatgtaccc
     3361 cgaggacggc gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca
     3421 ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc
     3481 ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3541 acagtacgag cgcgccgagg gccgccactc caccggcgcc taagcttaga gctcgaattt
     3601 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     3661 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     3721 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     3781 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     3841 atctatgtta ctagatcggg aattggttcc ggaaccaatt cgtaatcatg gtcatagctg
     3901 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3961 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc
     4021 gcctatttct attaattccc gatctagtaa catagatgac accgcgcgcg ataatttatc
     4081 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     4141 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     4201 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     4261 taagaaactt tattgccaaa tgtttgaacg atcggggaaa ttcgcctcga gtgacttctc
     4321 tgattgcaga ctatttatac caaccagcaa gaaagtgtcg aagtatgaag cagaaggcgc
     4381 aggctagagc ctggaaaacg agaagatgga tgcaaagaga cttttctgat ttaggaccaa
     4441 acaacctcaa gtaaacattg acgagtgtcc catcttttgt gccggtcagt aatccggtag
     4501 caggatcata ccttgggaga cgatggcgcg gacatttcac aatcccagca agctgaggaa
     4561 gcgataatag aaggttcaga acttgaggaa gaaagaagat gaggagggtt tcactgaagt
     4621 gacccagaat accaacaact gccatagtca ttccggcgaa gactgtgtaa gtgtctccaa
     4681 caaaaactga agaaggatac caattgtaag caagcattgc caaggatgtg gccatcagcg
     4741 gttgagtaag aaatatcgag aaggcatgag cttggtgata ctcgggatca acagatgctc
     4801 cgatttgcat aacgttgtgt atcaggatag cagcggcaat aacaactgtt tgaccgattt
     4861 caaggccatt cagaccagcg tggatgttaa tagagtttgt acaaaagaca gcaagtagcc
     4921 ccatatataa cttatatatc cttcctagat taagcacttc caagccaata taagcaacaa
     4981 gaggttttgg tataacaata gttgtatgac cagcataagc catcaaaagt ggaagggtag
     5041 caaaagatgg caggacaagt tttactctcc aaggcacatc aaggacatca tctacaaatc
     5101 caagcaaaat catgaagcaa atagatgcta gtgctgcatt atactccaca agccaattag
     5161 aatcttcagt gaagttaaaa tactgaaata ttatcgccac gatcaaaaag acaataccaa
     5221 caacaatacc caatgactct ggcactttaa tatctccttg aggcgtgcct cttttgttga
     5281 tatcaaaacc aaacatgtta cgtctcagaa cgtatctggc agtaacagga atcattttga
     5341 gagtgacgaa aaacccaacc aagctaagcc ccgcgttaat tagaatcgat cgcttgagtt
     5401 cattatcaac cttgtagtga aaacagagaa ggtatagata gagcgaacag agaagagtag
     5461 agatcacgaa gatcactcct aatttcggcg gcgcgagacg aaattcctca ccggaagcag
     5521 aaaccgtctt tcgggtcatc ttctgctccg atggcgctga gttgggttcc gacgggtccg
     5581 gtttgttggg aattgagatc gatgaagctc tcttacgagc tgccatatct tattgccccc
     5641 cgggatcaga tctggattga gagtgaatat gagactctaa ttggataccg aggggaattt
     5701 atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg
     5761 cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga
     5821 aacccgcggc tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg
     5881 cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc
     5941 ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
     6001 gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata
     6061 aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg
     6121 cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc
     6181 gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt
     6241 gcttgcggca gcgtgaagct gatccgtcga gattaataga aataaattca gcctaattcg
     6301 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     6361 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     6421 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     6481 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     6541 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     6601 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc
     6661 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     6721 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     6781 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     6841 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     6901 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     6961 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     7021 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     7081 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     7141 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     7201 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     7261 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     7321 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     7381 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     7441 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     7501 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     7561 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     7621 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     7681 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg
     7741 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
     7801 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
     7861 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7921 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7981 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8041 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     8101 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8161 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8221 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8281 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8341 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8401 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8461 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8521 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8581 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8641 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8701 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     8761 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8821 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     8881 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     8941 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     9001 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     9061 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     9121 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     9181 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     9241 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     9301 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     9361 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     9421 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     9481 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     9541 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     9601 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     9661 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     9721 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     9781 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     9841 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     9901 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     9961 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
    10021 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
    10081 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
    10141 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
    10201 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
    10261 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
    10321 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
    10381 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
    10441 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
    10501 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
    10561 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt
    10621 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
    10681 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
    10741 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
    10801 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
    10861 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
    10921 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
    10981 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
    11041 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
    11101 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
    11161 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
    11221 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
    11281 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
    11341 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
    11401 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
    11461 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
    11521 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
    11581 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
    11641 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
    11701 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
    11761 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
    11821 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
    11881 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
    11941 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
    12001 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
    12061 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
    12121 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
    12181 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
    12241 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
    12301 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
    12361 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
    12421 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
    12481 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
    12541 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
    12601 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
    12661 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
    12721 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
    12781 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
    12841 t
//
